Immunoaffinity Chromatography of s-Triazines

Abstract

Immunoaffinity chromatography (IAC) provides a selective method for sample preparation prior to high-performance liquid chromatography (HPLC) or gas chromatography (GC). Five different support materials were tested for their suitability with regard to IAC of herbicides (s-triazines). Three gels showing low (0-10 %) nonspecific s-triazine adsorption were used as affinity supports (Beaded cellulose ONB-carbonate A, LiChroprep hydrazide tentacle gel and LiChroprep azlactone gel). A monoclonal antibody (K4E7), directed against atrazine, was covalently coupled to the gels. High antibody immobilization rates (100 %) were obtained with the beaded cellulose gel in comparison to the other gels (60-70 %). Subsequently the IAC columns were loaded with triazine solutions. Glycine-HCl 0.2 mol/L, pH 2.2 yielded the best results for the elution of the bound triazines from the beaded cellulose IAC columns (70-100 %). Both the hydrazide tentacle and the azlactone IAC columns showed lower elution rates (60-80 %).