A chemiluminescence immunoassay for evaluation ofCryptosporidium parvumgrowth in vitro
Open Access
- 1 March 1996
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 136 (3), 251-256
- https://doi.org/10.1111/j.1574-6968.1996.tb08057.x
Abstract
A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 × 104 cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with 2 × 104 parasites/well. Two compounds (paromomycin and maduramicin) were evaluated and shown to have selective activity against C. parvum in a dose-dependent manner. There was excellent correlation between CLIA and immunofluorescence assay when assessing anti-C. parvum agents in MDCK cells. CLIA offers advantages over conventional enzyme-linked immunosorbent assay and immunofluorescence assay methods in that it is more sensitive and efficient. The combination of CLIA and MDCK culture provides an efficient tool for evaluating potential anti-cryptosporidial compounds prior to testing in animal models.Keywords
This publication has 6 references indexed in Scilit:
- Evaluation of maduramicin and alborixin in a SCID mouse model of chronic cryptosporidiosisAntimicrobial Agents and Chemotherapy, 1995
- Development of a microtitre ELISA to quantify development ofCryptosporidium parvumin vitroFEMS Microbiology Letters, 1995
- Cryptosporidium and cryptosporidiosis in man and animalsInternational Journal for Parasitology, 1995
- A Comparison Of Anticryptosporidial Activity Of Paromomycin With That Of Other Aminoglycosides And Azithromycin In Immunosuppressed RatsThe Journal of Infectious Diseases, 1994
- Multiple drug effect analysis with confidence intervalAntiviral Research, 1994
- Ultrasensitive immunoassay techniquesClinical Biochemistry, 1993