The Change of Bone Metabolism in Ovariectomized Rats : Analyses of MicroCT Scan and Biochemical Markers of Bone Turnover
Open Access
- 1 January 2012
- journal article
- Published by Korean Neurosurgical Society in Journal of Korean Neurosurgical Society
- Vol. 51 (6), 323-327
- https://doi.org/10.3340/jkns.2012.51.6.323
Abstract
The purpose of this study was to verify the appropriateness of ovariectomized rats as the osteoporosis animal model. Twelve female Sprague-Dawley rats underwent a sham operation (the sham group) or bilateral ovariectomy [the ovariectomy (OVX) group]. Eight weeks after operations, serum biochemical markers of bone turnover were analyzed; osteocalcin and alkaline phosphatase, which are sensitive biochemical markers of bone formation, and C-terminal telopeptide fragment of type I collagen C-terminus (CTX), which is a sensitive biochemical marker of bone resorption. Bone histomorphometric parameters and microarchitectural properties of 4th lumbar vertebrae were determined by micro-computed tomographic (CT) scan. The OVX group showed on average 75.4% higher osteocalcin and 72.5% higher CTX levels than the sham group, indicating increased bone turnover. Micro-CT analysis showed significantly lower bone mineral density (BMD) (p=0.005) and cortical BMD (p=0.021) in the OVX group. Furthermore, the OVX group was found to have a significantly lower trabecular bone volume fraction (p=0.002). Our results showed that bone turnover was significantly increased and bone mass was significantly decreased 8 weeks after ovariectomy in rats. Thus, we propose that the ovariectomized rat model be considered a reproducible and reliable model of osteoporosis.Keywords
This publication has 20 references indexed in Scilit:
- Influence of ferutinin on bone metabolism in ovariectomized rats. II: Role in recovering osteoporosisJournal of Anatomy, 2010
- Bone Mineral Density Changes after Ovariectomy in Rats as an Osteopenic Model : Stepwise Description of Double Dorso-Lateral ApproachJournal of Korean Neurosurgical Society, 2010
- Ovariectomy‐associated changes in bone mineral density and bone marrow haematopoiesis in ratsInternational Journal of Experimental Pathology, 2009
- The combination therapy with alfacalcidol and risedronate improves the mechanical property in lumbar spine by affecting the material properties in an ovariectomized rat model of osteoporosisBMC Musculoskeletal Disorders, 2009
- Influence of ferutinin on bone metabolism in ovariectomized rats. I: role in preventing osteoporosisJournal of Bone and Mineral Metabolism, 2009
- Genetic Variation in Structure-Function Relationships for the Inbred Mouse Lumbar Vertebral BodyJournal of Bone and Mineral Research, 2005
- Assessing bone quality — Animal models in preclinical osteoporosis researchBone, 1995
- Available animal models of osteopenia — Small and largeBone, 1995
- FDA guidelines and animal models for osteoporosisBone, 1995
- Osteoporosis and exerciseBone, 1995