Effect of D‐600 on inhibition of in vitro renin release in the rat by high extracellular potassium and angiotensin II.
- 1 July 1980
- journal article
- research article
- Published by Wiley in The Journal of Physiology
- Vol. 304 (1), 449-458
- https://doi.org/10.1113/jphysiol.1980.sp013335
Abstract
1. Renin was secreted by rat renal cortical slices incubated at 37 degrees C in a physiological saline solution. 2. Secretion was nearly abolished by incubation in a medium containing 60 mM‐K. Secretion could be restored to the control level by the addition of 5 X 10(‐7) M‐D‐600 (methoxy verapamil) to 60 mM‐K medium. 3. Angiotensin II inhibited renin secretion in a concentration‐dependent manner. Concentrations of D‐600 ranging from 1 to 3 X 10(‐6) M (two to sixfold higher than required to block the inhibitory effect of high K) failed to antagonize the inhibitory effect of angiotensin II. 4. Ca is required for the inhibitory effect of angiotensin II, however, as Ca‐depletion (incubation of slices in a medium with Na2EGTA and no added CaCl2)( progressively decreased and finally abolished any inhibitory effect. 5. These results confirm and extend previous observations suggesting that Ca plays an inhibitory coupling role in the control of renin secretion from the juxtaglomerular apparatus. Moreover, they suggest that although voltage‐sensitive channels exist on juxtaglomerular cells, angiotensin II activates an independent pathway for Ca mobilization.This publication has 22 references indexed in Scilit:
- Renin release by rat kidney slices incubated in vitro. Role of sodium and of alpha- and beta-adrenergic receptors, and effect of vincristine.Circulation Research, 1976
- Effect of Intrarenal Arterial Infusion of Magnesium on Renin Release in DogsExperimental Biology and Medicine, 1976
- Membrane potential of juxtaglomerular cellsNature, 1976
- Calcium and angiotensin tachyphylaxis in rat uterine smooth muscleAmerican Journal of Physiology-Legacy Content, 1975
- The sodium-potassium adenosine triphosphatase: pharmacological, physiological and biochemical aspects.1975
- The Influence of Ouabain on in vitro Renin Secretion and Intracellular SodiumNephron, 1975
- Effect of ouabain on renin secretion in anaesthetized dogsThe Journal of Physiology, 1974
- The Influence of Ouabain on in Vitro Renin SecretionExperimental Biology and Medicine, 1974
- CALCIUM DEPENDENCE OF THE INHIBITORY EFFECT OF ANGIOTENSIN ON RENIN SECRETION IN THE ISOLATED PERFUSED KIDNEY OF THE RATBritish Journal of Pharmacology, 1974
- FINE STRUCTURE OF NORMAL HUMAN JUXTAGLOMERULAR CELLS .I. GENERAL STRUCTURE AND INTERCELLULAR RELATIONSHIPS1966