PROBLEMS IN THE BIO-ASSAY OF ORALLY ADMINISTERED CEPHALOGLYCIN IN BIOLOGICAL FLUIDS AND METHOD FOR THE DETECTION OF ITS METABOLITE, DESACETYLCEPHALOGLYCIN

Abstract

Disagreements in serum and urine cephaloglycin determinations by Sarcina lutea with those by Bacillus subtilis or Streptococcus hemolyticus after oral administration of the antibiotic have been clarified by the present studies, attention being focussed on the formation of an active metabolite, desacetylcephaloglycin. The formation of desacetylcephaloglycin was detected by paper and thin-layer chromatography. Desacetylcephaloglycin was 0.1 to 0.5 as active as cephaloglycin against B. subtilis and S. hemolyticus but of comparable activity- against S. lutea PCI-1001.