Biochemical and Genetic Studies of Recombination Proficiency in Escherichia coli , I. Enzymatic Activity Associated with recB + and recC + Genes
- 1 April 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 65 (4), 955-961
- https://doi.org/10.1073/pnas.65.4.955
Abstract
An adenosine triphosphate-dependent deoxyribonuclease activity has been detected in lysates of recB(+)recC(+) strains. Mutations in recB or recC lead to loss of this activity, suggesting that these two genes determine the nuclease activity. The over-all reaction in crude lysates digests native DNA to nucleoside monophosphates. Complementation between recB21 and recC22 in vivo leads to normal levels of ATP-dependent nuclease activity. No complementation in vitro has been detected. Mutations in a third recombination gene (recA) do not alter significantly the wild-type levels of this nuclease activity.Keywords
This publication has 12 references indexed in Scilit:
- Endonuclease I-deficient and ribonuclease I-deficient Escherichia coli mutantsJournal of Molecular Biology, 1968
- Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.Proceedings of the National Academy of Sciences, 1968
- The General Recombination System of BacteriophageCold Spring Harbor Symposia on Quantitative Biology, 1968
- Enzymatic DNA Degradation in E. coli: Its Relationship to Synthetic Processes at the Chromosome LevelCold Spring Harbor Symposia on Quantitative Biology, 1968
- Revised linkage map of Escherichia coli.1967
- Lysis of Escherichia coli with a neutral detergentBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- The beginning of a genetic analysis of recombination proficiencyJournal of Cellular Physiology, 1967
- Formation of covalent circles of lambda DNA by E. coli extracts.Proceedings of the National Academy of Sciences, 1967
- Abnormal metabolic response to ultraviolet light of a recombination deficient mutant of Escherichia coli K12Journal of Molecular Biology, 1966
- The deoxyribonucleases of Escherichia coli. I. Purification and properties of a phosphodiesterase.1960