Dominant lethal gene test of 5-thio-D-glucose in male mice

Abstract

Genetic effects of 5-thio-D-glucose (5-thio) were tested in male mice by the dominant lethal gene test. Males were treated with phosphate buffered saline (PBS) or 5-thio dissolved in PBS by oral intubation daily at 40 or 60 mg/kg for 7 or 35 successive days. Average implantations per pregnant female and the intrauterine postimplantation losses in females mated with 5-thio treated males did not show any significant differences when compared statistically with parallel controls. Furthermore, the compound had no effect on the appearance and mating behavior of the male mice. This is the first report on the genetic effects of 5-thio to show that the compound in the treatment regimens studied were not mutagenic in terms of the induction of dominant lethal mutation in male mice.