Fucosylation of Xyloglucan: Localization of the Transferase in Dictyosomes of Pea Stem Cells
- 1 July 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 84 (3), 753-756
- https://doi.org/10.1104/pp.84.3.753
Abstract
Microsomal membranes from elongating regions of etiolated Pisum sativum stems were separated by rate-zonal centrifugation on Renografin gradients. The transfer of labeled and xylose from GDP-[14C]fucose and UDP-[14C]xylose to xyloglucan occurred mainly in dictyosome-enriched fractions. No transferase activity was detected in secretory vesicle fractions. Pulse-chase experiments using pea stem slices incubated with [3H]fucose suggest that xyloglucan chains are fucosylated and their structure completed within the dictyosomes, before being transported to the cell wall by secretory vesicles.This publication has 15 references indexed in Scilit:
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