Metabolic Abnormalities of 2,6-Diaminopurine-Resistant Mutants of Salmonella typhimurium.

Abstract

In a mutant resistant to 2, 6-diaminopurine (DAP) the mechanisms of an abnormal excretion of adenine was examined as a possible faulty feedback control. Feedback inhibition of purine biosynthesis is normal in this mutant as indicated by inhibition of synthesis of a purine precursor by adenine, adenosine and hypoxanthine. More adenine was required for feedback inhibition of the mutant strain, but these differences are relative to poorer utilization of adenine.