Somatic neurons in the brainstem and neocortex projecting to the external nucleus of the inferior colliculus: An anatomical study in the opossum

Abstract

Some brainstem and neocortical neurons projecting to the external nucleus of the inferior colliculus were identified by retrograde axonal transport of horseradish peroxidase (HRP). Large pressure injections of HRP were used to define the population of cells in the dorsal column and trigeminal nuclei projecting to the entire inferior colliculus. Subsequently, tiny injections of HRP were delivered electrophoretically to various parts of the external nucleus using micropipettes. In each case the electrophoretic placements were made in sites identified as somatic by their response to foot shock or manual stimulation of the body. Receptive fields were found to be large and almost always bilaterally symmetric. Areas excitable by stimulation of the upper body were often found adjacent to areas excitable by stimulation of the lower body, tail, or, rarely, the face and head. In general, sites in the external nucleus which were identified by their responsiveness to stimulation of the body were not responsive to click stimuli. No topographic representation of the body was evident. After small electrophoretic injections of HRP absolutely restricted to the external nucleus, HRP-filled neurons were found bilaterally in the nucleus gracilis, nucleus Bischoff, (the midline and tail representation of the dorsal column nuclei), nucleus cuneatus, and nucleus trigeminalis, subnucleus caudalis. Ipsilateral neocortical cellular filling was found primarily in a continuous strip along the caudal and inferior margin of the parietotemporal cortex, in the area corresponding to SMII (Pubols, '77). Rarely, filled cells were found in the parietal area corresponding to SMI and in the temporal pole. In sum, small somaticactivated loci of the external nucleus receive bilateral, topographically convergent projections from hindbrain somatosensory structures and from the SMII cortex.