The prosthetic group of cytochrome oxidase. 1. Purification as porphyrin a and conversion into haemin a
- 1 April 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 78 (4), 793-797
- https://doi.org/10.1042/bj0780793
Abstract
A method of purifying porphyrin a of high spectroscopic purity and approximately 85% absolute purity from ox heart is described and the method given for its conversion into haemin a. During the conversion of haemin a or other formyl haemins into their respective porphyrins, special treatment of the acetic acid solvent is often necessary to prevent oxidation of the formyl group to carboxyl.Keywords
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