Nonhistone Proteins Control Gene Expression in Reconstituted Chromatin
Open Access
- 1 December 1974
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (12), 5057-5061
- https://doi.org/10.1073/pnas.71.12.5057
Abstract
Chromatin was reconstituted from the purified DNA and histones of chicken erythrocytes and the nonhistone proteins of either chicken reticulocytes or chicken liver. Reconstituted chromatins, native chicken reticulocyte chromatin, and free DNA were transcribed with Escherichia coli RNA polymerase and the concentrations of globin-specific sequences in the RNA products were measured by hybridization with [3H]DNA complementary to chicken globin messenger RNA. Reticulocyte, but not liver, nonhistone proteins were shown to activate the globin genes in reconstituted erythrocyte chromatin. The transcripts of native and reconstituted chromatins were indistinguishable in respect of both the total yield of the RNA and the fractional yield of globin-specific sequences.Keywords
This publication has 29 references indexed in Scilit:
- Cell-Free Transcription of Mammalian Chromatin: Transcription of Globin Messenger RNA Sequences from Bone-Marrow Chromatin with Mammalian RNA PolymeraseProceedings of the National Academy of Sciences, 1974
- Isolation of High‐Molecular‐Weight DNA from Mammalian CellsEuropean Journal of Biochemistry, 1973
- Globin Messenger Sequences in High Molecular Weight RNA from Embryonic Mouse LiverNature New Biology, 1973
- Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-CelluloseProceedings of the National Academy of Sciences, 1972
- In vitro Synthesis of DNA Complementary to Rabbit Reticulocyte 10S RNANature New Biology, 1972
- Molecular Weight Determination of Nucleic Acids by Gel Electrophoresis in Non-aqueous SolutionNature New Biology, 1972
- An Electrophoretic Comparison of Vertebrate HistonesJournal of Biological Chemistry, 1971
- Role of non‐histone components in determining organ specificity of rabbit chromatinsFEBS Letters, 1970
- Isolation and characterization of nuclei from bird erythrocytesExperimental Cell Research, 1969
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965