A monoclonal antibody raised against adultSchistosoma mansoni which recognizes a surface antigen on schistosomula
- 1 January 1984
- journal article
- research article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 70 (2), 189-197
- https://doi.org/10.1007/bf00942221
Abstract
A monoclonal antibody has been raised by immunizing a mouse with an isolated tegumental preparation of adultSchistosoma mansoni. The hybridoma, designated NIMP/M.47, secreted an IgG2a antibody which was positive by indirect immunofluorescence with live schistosomula ofS. mansoni, but not with live schistosomula ofS. bovis, or with other living life cycle stages ofS. mansoni. In complement-dependent, or cell-mediated in vitro cytotoxicity assays, the monoclonal antibody mediated levels of schistosomular killing as high as those obtained with sera from infected mice. No significant protection, however, was obtained in passive transfer experiments. NIMP/M.47 was specific for a 20,000 dalton polypeptide in the schistosomular surface, which was also recognized by serum from infected mice.Keywords
This publication has 27 references indexed in Scilit:
- Schistosoma mansoni: Acquired resistance of developing schistosomula to immune attack in vitroExperimental Parasitology, 1982
- Passive immunization of mice againstSchistosoma mansoniwith an IgM monoclonal antibodyParasitology, 1982
- Monoclonal antibodies against surface antigens of schistosomula of Schistosoma mansoniParasitology, 1982
- Isolation and partial characterization of the tegumental outer membrane of adultSchistosoma mansoniParasitology, 1981
- Recovery ofSchistosoma mansonifrom the skin, lungs and hepatic portal system of naive mice and mice previously exposed toS. mansoni: evidence for two phases of parasite attrition in immune miceParasitology, 1980
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Schistosoma mansoni: Defined system for stepwise transformation of cercaria to schistosomule in vitroExperimental Parasitology, 1974
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964