Bovine Lymphocyte Antigens (BoLA): A Serologic, Genetic and Histocompatibility Analysis

Abstract

Eleven lymphocyte antigens were defined in cattle using 81 lymphocytotoxic sera. These sera (typing reagents) were selected from over 1000 normal sera, 35 alloimmune sera and 111 samples of colostrum whey. Absorptions revealed that 9 of the 11 antigens detected by these reagents are serologically independent. Segregation of the genes controlling these antigens was observed among 470 calves from matings of 56 heterozygous sires and 360 dams, indicating that each of the 11 antigens is controlled by 1 of 11 alleles at a single autosomal co-dominant locus BoLA-A (Bovine Lymphocyte Antigen, locus A). To determine the relation between BoLA antigens and histocompatibility, skin allografts were exchanged among 4 unrelated adult cows. Incompatibilities for the SD [serologically defined] antigens, but not MLR [mixed lymphocyte response] or blood group antigens, were predictive of early rejection of allografts. Graft recipients produced antibodies against the SD antigens of incompatible donors. The BoLA-A locus apparently is part of the major histocompatibility complex (MHC) of cattle.