The In Vitro14C-Cyclic AMP Production by Normal Human Adipose Tissue in Response to Some Hormones and in Uncontrolled and Controlled Diabetic Adipose Tissue

Abstract

The ¹⁴C-c-AMP production by human subcutaneous adipose tissue biopsies has been measured in response to noradrenaline and in groups of 12 uncontrolled and 9 insulin-controlled diabetics and contrasted with that of 10 non-diabetic controls obtained during an operation. With in vitro incubated normal human adipose tissue, measurements following the addition of noradrenaline showed a tenfold increase in the cellular content of ¹⁴C-c-AMP at 10-40 min after the noradrenaline, and preceding their release of glycerol. Addition of insulin to the incubate prevented both these effects. Subcutaneous adipose tissue from uncontrolled diabetics showed increased adenyl cyclase activity and decreased phosphodiesterase activity, while tissue from insulin-controlled diabetics showed these enzyme activities in the normal range.