Surface Plasmon Resonance Intensity in Ex Situ Analysis of Protein Arrays Using a Wavelength Interrogation-Based Surface Plasmon Resonance Sensor
- 1 May 2004
- journal article
- Published by IOP Publishing in Japanese Journal of Applied Physics
- Vol. 43 (5R)
- https://doi.org/10.1143/jjap.43.2756
Abstract
We have investigated the properties of the surface plasmon resonance (SPR) spectra obtained by scanning each spot of protein arrays using a wavelength interrogation-based SPR sensor (custom-made). The surface structure of each protein array consists of glass/metal/proteins/air (ex situ). The analysis of serial spectra showed marked increases in SPR intensity and wavelength at the boundary area of protein array spots, which were mainly caused by the background signal from the glass substrate. However, at the central area, protein interaction caused an increase in SPR wavelength, and a decrease in SPR intensity. There was no significant difference between the central and boundary areas in terms of the roughness and thickness of the gold film. These results suggest that SPR intensity is a good indicator for distinguishing psuedosignals from real signals of biospecific interactions in the analysis of protein arrays using wavelength interrogation-based SPR sensors.Keywords
This publication has 13 references indexed in Scilit:
- Characterization of surface plasmon resonance wavelength by changes of protein concentration on protein chipsSensors and Actuators B: Chemical, 2003
- Investigation of Selective Protein Immobilization on Charged Protein Array by Wavelength Interrogation-based SPR SensorMolecules and Cells, 2003
- Protein chip technologyCurrent Opinion in Chemical Biology, 2002
- Global Analysis of Protein Activities Using Proteome ChipsScience, 2001
- Simultaneous Detection of Multiple Cytokines from Conditioned Media and Patient's Sera by an Antibody-Based Protein Array SystemAnalytical Biochemistry, 2001
- Dispersive surface plasmon microscopy for the characterization of ultrathin organic filmsApplied Physics Letters, 1996
- Principles of biosensing with an extended coupling matrix and surface plasmon resonanceSensors and Actuators B: Chemical, 1993
- Surface plasmon-polaritons and their usesOptical and Quantum Electronics, 1991
- Surface plasma oscillations at silver surfaces with thin transparent and absorbing coatingsSurface Science, 1978
- Surface electromagnetic waves ellipsometrySurface Science, 1976