Cold synchronization for the study of peripheral blood and bone marrow chromosomes in leukemias and other hematologic disease states
- 1 May 1980
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 54 (2), 207-211
- https://doi.org/10.1007/bf00278974
Abstract
A method of cold-shock synchronization of immature granulocytic cells from peripheral blood or bone marrow is described. It is shown that this method provides an increased yield of early metaphases and offers advantages over others currently employed. The peaks of mitotic activity following the cold-shock treatment differ for patients with acute non-lymphoblastic leukemia (ANLL) and patients with chronic myeloid leukemia (CML) by an interval of 2 h. This method is considered to be suitable for routine cytogenetic studies on hematological patients.This publication has 6 references indexed in Scilit:
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- SYNCHRONIZATION OF MAMMALIAN CELLS WITH RESPECT TO THE MITOTIC CYCLE*Cell Proliferation, 1971
- Effects of Temperature on the Mitotic Cycle of Normal and Synchronized Mammalian CellsPublished by Elsevier ,1966