Ion Exchange Separation of Fructose-1-Phosphate Using Borate as Eluant

Abstract

Ion exchange separation of sugar phosphates by using dilute borate elution as eluant has been established by K h y m et al. (1-3). Separation of fructose-l-phosphate (F-l-P), however, has not been reported in their papers. As the eluting agents, 1×10^-3M K₂B₄O₇ was used for the elution of glucose-6-phosphate (G-6-P) and l×lO^-5M K₂B.O₇ for that of fructose-6-phosphate (F-6-P), but 1×10^-4M K₂B₄O₇ has not been used in their procedure. This communication describes that 1×10^-4M borate elution gave a good separation of F-l-P from other sugar phosphates.