Effects of Glucagon-Like Peptide 1 (7-36) Amide and Glucagon on Amylin Release from Perfused Rat Pancreas

Abstract

The effects of glucagon-like peptide 1 (7-36) amide [GLP-1(7-36)amide] and glucagon on the release of islet amyloid polypeptide (IAPP), or amylin, from the isolated perfused rat pancreas were studied. In the presence of 5.6 mM glucose, GLP-1 (7-36) amide and glucagon stimulated the release of amylin from the perfused pancreas. The infusion of GLP-1 (7-36) amide at a concentration of 10^-9M elicited a biphasic release of amylin similar to that of insulin. The cumulative output of amylin induced by 10^-9M GLP-1 (7-36) amide was significantly higher than that by 10^-9M glucagon (p < 0.01). The amylin/insulin molar ratios induced by GLP-1 (7-36) amide and glucagon were about 1% and did not differ significantly. These findings suggest that GLP-1 (7-36) amide and glucagon stimulate the release of amylin from the pancreas and that the concomitant secretion of amylin and insulin might contribute to glucose homeostasis.