Distribution of several proteases inside and outside the central vacuole of Chara australis.
- 31 December 1985
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 11 (1), 81-86
- https://doi.org/10.1247/csf.11.81
Abstract
Vacuolar sap was separated by intracellular perfusion and the distribution of proteases was studied in the giant alga C. australis. Caseinolytic and hemoglobin-digesting activities were found to be higher at low pH, and about 85% of total activity was localized in the central vacuole. The optimal pH for carboxypeptidase activity measured using N-carbobenzoxy-L-phenylalanyl-L-alanine as a substrate was around 5, and about 95% of total activity was localized in the vacuole. Moreover a substantial portion (40%) of aminopeptidase activity, measured at pH 5.5 using L-leucine-.beta.-naphthylamide as a substrate, was found in the vacuole.This publication has 9 references indexed in Scilit:
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