Influence of Hemagglutinating Viruses on Tumor Cell Suspensions: I. Growth Inhibition and Reversal of the Effect.

Abstract

When equal amts. of allantoic fluid infected with New castle Disease (NDV) virus were mixed with a 10% suspension of sarcoma 180 cells, inoculations of these mixtures subcut. into mice resulted in failure of the cells to grow. Control tumor suspensions, mixed with normal allantoic fluid under the same conditions, grow. The reaction, which was associated with a fall in hemagglutination (HA) titer of the virus took place immediately and was apparent whether the mixtures were incubated at 4[degree]C or room temp. When mixtures of virus and tumor cells were allowed to stand at room temp. for periods of 1-3 hrs., either after 4[degree] or continuously, inoculation of the mixtures resulted in tumor growth. This was usually associated with a rise in HA titer. When large quantities of cells {20% suspensions) were used or when the virus was diluted 1-1000, tumor inhibition did not occur. The reaction appeared to be related to the number of tumor cells present and the amt. of virus in the mixtures. The tumor inhibition could be reversed by specific immune serum. NDV multiplication in sarcoma 180 inthe mouse and also in sarcoma 180 tissue culture could not be demonstrated. The results are considered as being analogous to the adsorption and elution of the virus on red blood cells. Other viruses, PR8 influenza and WS neuro-influenza, have shown similar activities with the sarcoma 180 suspensions. Ehrlich tumor suspensions also gave this type of reaction with NDV.