Gas Liquid Chromatographic—Mass Spectrometric Methods for Quantitation of Prostaglandin Analogs

Abstract
The synthesis of tetradeuterated 15-methyl-PGF and 17-phenyl-18, 19, 20-trinor-PGF, is described. Gas chromatographic -mass spectrometric methods, using the deuterated compounds as internal standards and carriers, for quantitation of corresponding unlabeled prostaglandin analogues is described. With acceptable accuracy and precision the sensitivity is in the subpicomole range.