A Protein Kinase Substrate Identified by the Two-Hybrid System
- 31 July 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 257 (5070), 680-682
- https://doi.org/10.1126/science.1496382
Abstract
A genetic method, the two-hybrid system, was used to identify four genes encoding proteins that interact with the SNF1 protein kinase from yeast. One of the genes, SIP1, was independently isolated as a multicopy suppressor of defects caused by reduced SNF1 kinase activity, and genetic evidence supports its function in the SNF1 pathway. The SIP1 protein co-immunoprecipitated with SNF1 and was phosphorylated in vitro. Thus, the two-hybrid system, which is applicable to any cloned gene, can be used to detect physical interactions between protein kinases and functionally related substrate proteins.Keywords
This publication has 14 references indexed in Scilit:
- Yeast casein kinase I homologues: an essential gene pair.Proceedings of the National Academy of Sciences, 1992
- Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein kinase cDNA.Proceedings of the National Academy of Sciences, 1991
- Generating yeast transcriptional activators containing no yeast protein sequencesNature, 1991
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Mutants of GAL4 protein altered in an activation functionCell, 1987
- A Yeast Gene That Is Essential for Release from Glucose Repression Encodes a Protein KinaseScience, 1986
- Regulation of the Yeast HO GeneCold Spring Harbor Symposia on Quantitative Biology, 1985
- [10] Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeastMethods in Enzymology, 1983
- Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertaseCell, 1982
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977