PROPERTIES OF ARABINONUCLEIC ACIDS (ANA & 20′F-ANA): IMPLICATIONS FOR THE DESIGN OF ANTISENSE THERAPEUTICS THAT INVOKE RNASE H CLEAVAGE OF RNA
- 31 March 2001
- journal article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 20 (4-7), 429-440
- https://doi.org/10.1081/ncn-100002317
Abstract
Inversion of configuration of the C2′ position of RNA leads to a very unique nucleic acid structure: arabinonucleic acid (ANA). ANA, and its 2′-fluoro derivative (2′ F-ANA) form hybrids with RNA that are capable of activating RNase H, resulting in cleavage of the RNA strand. In this paper, we review the properties of duplexes formed between ANA (or 2′F-ANA) and its RNA complement. These studies support the notion that RNase H is sensitive to the minor groove dimensions of the hybrid substrate.Keywords
This publication has 38 references indexed in Scilit:
- Synthesis and Biophysical Properties of Arabinonucleic Acids (ANA): Circular Dichroic Spectra, Melting Temperatures, and Ribonuclease H Susceptibility of ANA·RNA Hybrid DuplexesBiochemistry, 2000
- Triple helices containing arabinonucleotides in the third (Hoogsteen) strand: effects of inverted stereochemistry at the 2'-position of the sugar moietyNucleic Acids Research, 1998
- D-2-Deoxyribose and D-Arabinose, but not D-Ribose, Stabilize the Cytosine Tetrad (i-DNA) StructureJournal of Biomolecular Structure and Dynamics, 1997
- Effects of Arabinosylcytosine-Substituted DNA on DNA/RNA Hybrid Stability and Transcription by T7 RNA PolymeraseBiochemistry, 1994
- Nucleosides. Part LV. Efficient synthesis of arabinoguanosine building blocksHelvetica Chimica Acta, 1994
- Hybridization properties of oligoarabinonucleotidesCanadian Journal of Chemistry, 1994
- Deoxyribonucleotide analogs as inhibitors and substrates of DNA polymerasesPharmacology & Therapeutics, 1990
- Solution and solid phase chemical synthesis of arabinonucleotidesCanadian Journal of Chemistry, 1989
- Functional consequences of the arabinosylcytosine structural lesion in DNABiochemistry, 1988
- The rapid chemical synthesis of arabinonucleotidesTetrahedron Letters, 1987