Developmental Changes in Glycolipid Synthesizing Enzymes in the Brain of a Myelin‐Deficient Mutant Wistar Rat
- 1 November 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (5), 1434-1439
- https://doi.org/10.1111/j.1471-4159.1982.tb12588.x
Abstract
Changes in the activities of UDP-galactose:ceramide galactosyltransferase (CGalT, EC 2.4.1.45), UDP-glucose:ceramide glucosyltransferase (CGlcT, EC 2.4.1.80) and 3''-phosphoadenosine-5''-phosphosulfate (PAPS): galactosylceramide 3''-sulfotransferase (EC 2.8.2.11) over the myelinating period between 12 and 25 days were studied in the brains of control and myelin-deficient rats. Although the activity of galactosyltransferase with ceramides containing hydroxy fatty acids quadrupled in normal male littermates between 14 and 20 days, hardly any increase was observed in the mutant and the activity was < 10% of control above 20 days of age. With normal fatty acid containing ceramides as acceptors, the activity decreased from 83% of the control at 12 days to .apprx. 30% after 20 days. Sulfotransferase activity also did not show the normal increase during the 3rd wk of life and declined from 60 to 22%. Glucosyltransferase and lysosomal hydrolases in brain and ceramide galactosyltransferase in sciatic nerves appeared normal. Close similarities to the jimpy mutant mouse in which myelin deficiency is also inherited as an x-linked recessive trait are suggested.Keywords
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