DNA synthesis arrest sites at the right terminus of rat long interspersed repeated (LINE or LIRn) DNA family members
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (7), 3155-3175
- https://doi.org/10.1093/nar/15.7.3155
Abstract
An approximately equal to 150-bp GC-rich (approximately equal to 60%) region is at the right end of rat long interspersed repeated DNA (LINE or L1Rn) family members. We report here that one of the DNA strands from this region contains several non-palindromic sites that strongly arrest DNA synthesis in vitro by the prokaryotic Klenow and T4 DNA polymerases, the eukaryotic alpha polymerase, and AMV reverse transcriptase. The strongest arrest sites are G-rich (approximately equal to 70%) homopurine stretches of 18 or more residues. Shorter homopurine stretches (12 residues or fewer) did not arrest DNA synthesis even if the stretch contains 11/12 G residues. Arrest of the prokaryotic polymerases was not affected by their respective single strand binding proteins or polymerase accessory proteins. The region of duplex DNA which contains DNA synthesis arrest sites reacts with bromoacetaldehyde when present in negatively supercoiled molecules. By contrast, homopurine stretches that do not arrest DNA synthesis do not react with bromoacetaldehyde. The presence of bromoacetaldehyde-reactive bases in a G-rich homopurine-containing duplex under torsional stress is thought to be caused by base stacking in the homopurine strand. Therefore, we suggest that base-stacked regions of the template arrest DNA synthesis.Keywords
This publication has 55 references indexed in Scilit:
- L1 family of repetitive DNA sequences in primates may be derived from a sequence encoding a reverse transcriptase-related proteinNature, 1986
- Salt-dependent changes in the DNA binding co-operativity of Escherichia coli single strand binding proteinJournal of Molecular Biology, 1986
- Conservation throughout mammalia and extensive protein-encoding capacity of the highly repeated DNA long interspersed sequence oneJournal of Molecular Biology, 1986
- Poly(dG)-poly(dC) sequences, under torsional stress, induce an altered DNA conformation upon neighboring DNA sequencesCell, 1985
- The role of palindromic and non-palindromic sequences in arresting DNA synthesis in vitro and in VivoJournal of Molecular Biology, 1984
- Dispersal process associated with the L1 family of interspersed repetitive DNA sequencesJournal of Molecular Biology, 1984
- Site-specific pausing of deoxyribonucleic acid synthesis catalyzed by four forms of Escherichia coli DNA polymerase IIIBiochemistry, 1983
- Organization and evolutionary progress of a dispersed repetitive family of sequences in widely separated rodent genomesJournal of Molecular Biology, 1981
- Termination of DNA replication in vitro at a sequence-specific replication terminusCell, 1981
- X-ray fiber diffraction and model-building study of polyguanylic acid and polyinosinic acidJournal of Molecular Biology, 1975