Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor.
- 1 October 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (19), 7216-7220
- https://doi.org/10.1073/pnas.83.19.7216
Abstract
Two endothelial cell growth factors (ECGF) have been purified from bovine brain and termed .alpha.- and .beta.-ECGF [Burgess, W.H., Mehlman, T., Friesel, R., Johnson, W. V. and Maciag, T. (1985) J. Biol. Chem. 260, 11389-11392]. Amino acid sequence analysis indicates that .beta.-ECGF represents a 20-amino acid amino-terminal extension of .alpha.-ECGF and a 14 amino acid amino-terminal extension of acidic fibroblast growth factor. These data indicate that both .alpha.-ECGF and acidic fibroblast growth factor may be derived from .beta.-ECGF by posttranslational processing. Analysis of the amino-terminal 14 residues of .beta.-ECGF by fast-atom-bombardment mass spectrometry established the amino acid sequence of this region and the identity of the blocking group at the amino terminus (acetyl).This publication has 23 references indexed in Scilit:
- Purification and characterization of heparin-binding endothelial cell growth factors.Journal of Biological Chemistry, 1986
- Brain-Derived Acidic Fibroblast Growth Factor: Complete Amino Acid Sequence and HomologiesScience, 1985
- Primary structure of bovine brain acidic fibroblast growth factor (FGF)Biochemical and Biophysical Research Communications, 1985
- A unique family of endothelial cell polypeptide mitogens: the antigenic and receptor cross-reactivity of bovine endothelial cell growth factor, brain-derived acidic fibroblast growth factor, and eye-derived growth factor-II.The Journal of cell biology, 1985
- Peptide studies using a fast atom bombardment high field mass spectrometer and data system. 4. Disulfide-containing peptidesJournal of Mass Spectrometry, 1985
- Bovine retina contains three growth factor activities with different affinity to heparin: eye derived growth factor I, II, IIIBiochimie, 1985
- Heparin affinity of anionic and cationic capillary endothelial cell growth factors: analysis of hypothalamus-derived growth factors and fibroblast growth factors.Proceedings of the National Academy of Sciences, 1985
- Culture of vascular endothelium.1976
- HUMAN VASCULAR ENDOTHELIAL CELLS IN CULTUREThe Journal of cell biology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970