Sensitization of algal virus to UV by the incorporation of 5-bromouracil and mutations of host algaPlectonema boryanum

Abstract

Ultraviolet light (UV) sensitivity and photoreactivation of algal virus (cyanophage) LPP-1 were studied after multiplication in host alga Plectonema boryanum in presence of 5-bromouracil (5-BU) alone and in conjunction with sulfanilamide. Virus particles containing 5-BU were more sensitive towards UV and also showed low photoreactivation. There was less incorporation of 5-BU in virus without pretreatment of host alga with sulfanilamide, an inhibitor of thymine synthesis. 5-BU-induced short trichome mutants of Plectonema boryanum were isolated. These mutants grew slowly in liquid medium as well as on agar plates and differed in other morphological characters. Reversion of short trichome mutants was observed with a frequency of about 10(-3), but revertants were different from parent alga. The short trichome mutants were sensitive to virus LPP-1 and resistant towards UV.