IL-4 production by T cells from naive donors. IL-2 is required for IL-4 production.
- 15 August 1990
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 145 (4), 1127-1136
- https://doi.org/10.4049/jimmunol.145.4.1127
Abstract
Utilizing a sensitive and selective assay for IL-4, it was shown that lymph node T cells from naive mice could produce small amounts of this lymphokine in response to anti-CD3 antibodies adsorbed to culture dishes. The capacity of these cells to produce IL-4 in response to plate-bound anti-CD3 was substantially enhanced by the addition of IL-2 to the culture and was strikingly inhibited by monoclonal anti-IL-2 antibody. Thus, IL-2 appears to be essential for IL-4 production by anti-CD3 antibody-stimulated T cells from naive mice. The effect of IL-2 was not mediated either by preferential proliferation or survival of precursors of IL-4 producing cells, indicating that IL-2 regulates T cell production of IL-4. IL-4 producing capacity of T cells from naive mice was found mainly among CD4+ T cells. Large T cells produced much more IL-4, on a per cell basis, than did small T cells. In contrast, small T cells appeared to be equal or superior to large T cells in producing IL-2. The superiority of large T cells in IL-4-producing capacity was not accounted for by a lack of an accessory cell population from the small T cells as addition of large spleen cells depleted of both B and T cells did not enhance IL-4 production by small lymph node T cells. These results suggest that the bulk of IL-4 production by T cell populations, from normal mice, in response to anti-CD3 depends upon cells that are already activated and that IL-2 is required for such production.This publication has 25 references indexed in Scilit:
- IgG2a restriction of murine antibodies elicited by viral infections.The Journal of Experimental Medicine, 1987
- Suppression of in vivo polyclonal IgE responses by monoclonal antibody to the lymphokine B-cell stimulatory factor 1.Proceedings of the National Academy of Sciences, 1986
- Purification to homogeneity of B cell stimulating factor. A molecule that stimulates proliferation of multiple lymphokine-dependent cell lines.The Journal of Experimental Medicine, 1986
- Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.The Journal of Immunology, 1986
- Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule.The Journal of Immunology, 1983
- Identification of a T cell-derived b cell growth factor distinct from interleukin 2.The Journal of Experimental Medicine, 1982
- IgD-secreting murine plasmacytomas: identification and partial characterization of two IgD myeloma proteins.The Journal of Immunology, 1981
- IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement.The Journal of Immunology, 1980
- Growth of factor-dependent hemopoietic precursor cell lines.The Journal of Experimental Medicine, 1980
- Rhesus Monkey B Lymphocyte Surface Immunoglobulin: Analysis with a Fluorescence-Activated Cell SorterThe Journal of Immunology, 1979