Reversible inhibition of mammalian glutamine synthetase by tyrosine nitration
Open Access
- 12 December 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 581 (1), 84-90
- https://doi.org/10.1016/j.febslet.2006.11.081
Abstract
The effect of tyrosine nitration on mammalian GS activity and stability was studied in vitro. Peroxynitrite at a concentration of 5 μmol/l produced tyrosine nitration and inactivation of GS, whereas 50 μmol/l peroxynitrite additionally increased S‐nitrosylation and carbonylation and degradation of GS by the 20S proteasome. (−)Epicatechin completely prevented both, tyrosine nitration and inactivation of GS by peroxynitrite (5 μmol/l). Further, a putative “denitrase” activity restored the activity of peroxynitrite (5 μmol/l)‐treated GS. The data point to a potential regulation of GS activity by a reversible tyrosine nitration. High levels of oxidative stress may irreversibly damage and predispose the enzyme to proteasomal degradation.Keywords
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