LEUKEMIC RETICULOENDOTHELIOSIS - HAIRY CELL LEUKEMIA, FUNCTIONAL AND STRUCTURAL FEATURES OF ABNORMAL CELL IN A PATIENT WITH PROFOUND LEUKOCYTOSIS

Abstract

The development of profound leukocytosis in a patient with leukemic reticuloendotheliosis (LRE) enabled isolation of purified LRE cells for the investigation of their structural and functional characteristics. The LRE cells of this patient bore surface immunoglobulin and had complement receptors but did not bear Fc receptors and did not form rosettes with sheep erythrocytes. By EM, the cells were observed to contain typical ribosome lamella structures and to phagocytize both 0.81 .mu.m latex particles and complement-coated zymosan particles. They were adherent to glass and nylon wool fibers. The mitogenic response to erythroagglutinating phytohemagglutinin was normal in magnitude but delayed chronologically. The binding of 125I-labeled plant lectins was used to characterize the surface topography of LRE cells. Results of these studies indicated that the LRE cell surface differed significantly from the surface of normal T [thymus-derived] and B [bone marrow-derived] lymphocytes and chronic lymphatic leukemia cells. The LRE cells were capable of stimulating and responding in a 1-way mixed lymphocyte culture. The LRE cells were not active as effector cells of cell mediated lympholysis, a T cell function, or antibody-dependent cellular cytotoxicity, a null cell function. They were effector cells of lectin-induced cellular cytotoxicity showing that they possessed the capacity to function as cytotoxic effector cells. These data indicated that LRE cells in this patient had surface and functional characteristics of lymphocytes and monocytes.