Avirulant Salmonella typhimurium strains prevent food allergy in mice
Open Access
- 9 January 2008
- journal article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 151 (3), 546-553
- https://doi.org/10.1111/j.1365-2249.2007.03582.x
Abstract
Oral tolerance to foods can be regulated by microorganisms in the gut lumen. We hypothesized that pretreatment with avirulent Salmonella typhimurium strains could prevent food allergy in mice. Mice were administered S. typhimurium PhoPc (STPhoPc) or S. typhimurium AroA prior to oral sensitization to β-lactoglobulin in the presence of cholera toxin. An oral antigen challenge after sensitization assessed antigen-induced anaphylaxis. Antigen-specific antibody titres were measured by enzyme-linked immunosorbent assay in the serum and enzyme-linked immunospot (ELISPOT) in the spleen, and cytokine-secreting cells were measured by ELISPOT in the Peyer's patches, lamina propria and epithelium cells. We showed first that S. typhimurium could up-regulate interleukin (IL)-12 and IL-10 secretion by gut T cells. Mice pretreated with STPhoPc had decreased anaphylaxis upon challenge, along with decreased immumoglobulin G1 (IgG1) and IgE antibody titres. Mice having received S. typhimurium AroA had partly decreased anaphylaxis as well as decreased serum IgG1 antibody titres in the serum, and increased serum IgA antibody titres. Antibody titres could be correlated with increased numbers of spleen and Peyer's patches antibody-producing cells. STPhoPc-treated mice showed significantly decreased anaphylaxis when compared with the control mice, while S. typhimurium AroA-pretreated mice had a similar immune response together with increased secretory IgA titres. Our experiments have proved a potential immunomodulatory protective effect by two avirulent S. typhimurium strains.Keywords
This publication has 28 references indexed in Scilit:
- AttenuatedSalmonella typhimuriumreduces ovalbumin-induced airway inflammation and T-helper type 2 responses in miceClinical and Experimental Immunology, 2006
- In search of a new paradigm: mechanisms of sensitization and elicitation of food allergy*Allergy, 2005
- The inverse association of salmonellosis in infancy with allergic rhinoconjunctivitis and asthma at school-age: a longitudinal studyAllergy, 2005
- Epidemiology of life‐threatening and lethal anaphylaxis: a reviewAllergy, 2005
- Antigen-specific secretory IgA antibodies in the gut are decreased in a mouse model of food allergyJournal of Allergy and Clinical Immunology, 2004
- Lymphocytes in Peyer patches regulate clinical tolerance in a murine model of food allergy☆Journal of Allergy and Clinical Immunology, 2004
- Allergy, Parasites, and the Hygiene HypothesisScience, 2002
- Bacterial virulence, proinflammatory cytokines and host immunity: how to choose the appropriate Salmonella vaccine strain?Microbes and Infection, 2001
- A murine model of IgE-mediated cow’s milk hypersensitivityJournal of Allergy and Clinical Immunology, 1999
- Family structure, neonatal infection, and hay fever in adolescence.Archives of Disease in Childhood, 1996