Antioxidant capacity of phenolic and related compounds: correlation among electrochemical, visible spectroscopy methods and structure–antioxidant activity
- 1 August 2001
- journal article
- Published by Taylor & Francis in Redox Report
- Vol. 6 (4), 243-250
- https://doi.org/10.1179/135100001101536391
Abstract
We investigated the antioxidant activity of phenylpropionic acids--caffeic (CAF), ferulic (FER), para-coumaric (COU) and cinnamic (CIN)--and phenolic acids and related compounds--gallic (GAL), methyl gallate (meGAL), vanillic (VAN) and gentisic (GEN)--using visible spectroscopy, inhibition of nitroblue tetrazolium (NBT) reduction, and electrochemical methods including cyclic voltammetry and potentiometry. In the spectroscopic assays, only CAF, GAL and meGAL were able to inhibit NBT reduction. The same compounds showed the lowest oxidation potentials (Epa) and the highest redox potentials deltaE) in the cyclic voltammetric and potentiometric studies, respectively. In addition, it was observed that the greater the number of hydroxyls linked to the aromatic ring, the greater was the antioxidant activity of the analysed compounds. The correlations of Spermann--used to compare the methods between themselves and the methods with the relationship structure-antioxidant activity--were r = -0.9762 for the cyclic voltammetric-potentiometric methods. r = 0.8333 for the inhibition of NBT reduction-potentiometric methods and r = -0.8095 for the inhibition of NBT reduction-cyclic voltammetric methods. The correlations for cyclic voltammetric, potentiometric and inhibition of NBT reduction methods-number of hydroxyls linked to the aromatic ring were r = -0.9636, 0.9636 and 0.9142, respectively. These findings indicate that the electrochemical methods together with spectroscopic studies are a good tool to evaluate the antioxidant activity of substances.Keywords
This publication has 29 references indexed in Scilit:
- Phenolics: Prooxidants or Antioxidants?Nutrition Reviews, 2009
- Urinary excretion of hydroxycinnamates and flavonoids after oral and intravenous administrationFree Radical Biology & Medicine, 1999
- Red wine, green tea and vitamins: Do their antioxidants play a role in immunologic protection against cancer or even AIDS?Biomedicine & Pharmacotherapy, 1999
- High Molecular Weight Plant Polyphenolics (Tannins) as Biological AntioxidantsJournal of Agricultural and Food Chemistry, 1998
- Antioxidant and Prooxidant Behavior of Flavonoids: Structure-Activity RelationshipsFree Radical Biology & Medicine, 1997
- Complexes of Iron with Phenolic Compounds from Soybean Nodules and Other Legume Tissues: Prooxidant and Antioxidant PropertiesFree Radical Biology & Medicine, 1997
- Antioxidant activities of flavonoids as bioactive components of foodBiochemical Society Transactions, 1996
- Iron coordination by catechol derivative antioxidantsBiochemical Pharmacology, 1996
- Polyphenolic Flavanols as Scavengers of Aqueous Phase Radicals and as Chain-Breaking AntioxidantsArchives of Biochemistry and Biophysics, 1995
- Antioxidant potential of intermediates in phenylpropanoid metabolism in higher plantsFEBS Letters, 1995