Linked scans of peptides and protein digests: Amino acid sequence determination of components of complex mixtures

Abstract

Two‐sector linked‐scan analysis of an unpurified proteolytic digest of a pyruvate decarboxylase enzyme (60 000 Da) has allowed the discovery and assignment of an amino‐terminal post‐translational modification and processing event. A difference in amino acid sequence from that predicted by a recently published nucleotide sequence has also been found. These results illustrate both the use and considerable potential of linked‐scan methods for the analysis of complex biopolymer mixtures.