The effects of sleep on neurons in isolated cerebral cortex

Abstract

Slabs of cat parietal cortex with some 2 mm of underlying white matter were surgically isolated from the rest of the nervous system, without interference with the superficial blood supply. Wire micro-recording electrodes were inserted into the isolated cortex; bone, muscle and skin wounds were repaired and the animal allowed to recover from anesthesia. The adequacy of surgical isolation was examined histologically 8-12 wk after operation. Only 1 of the 6 preparations reported here showed surviving neural connections with the rest of the brain. Soon after operation, spontaneous bursts of neural activity appeared within the isolated area. These became more frequent until neural discharge was continuous but irregular. Records were made from this time onwards. The interval distributions obtained from neurons within the isolated area did not differ significantly from log normal curves. When the unrestrained animal fell asleep, there was no significant alteration in the modal interval or geometric standard deviation of interval distributions recorded from cells in isolated cortex. The interval distributions of neurons in isolated cerebral cortex resembled those of neurons in the intact cortex of an alarmed animal. The reduction of modal interval that is shown by neurons in intact cortex when an animal falls asleep is probably due to the neural influence of infracortical structures.