Tumour‐promoting phorbol esters inhibit agonist‐induced phosphatidate formation and Ca2+ flux in human platelets
- 28 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 180 (2), 160-164
- https://doi.org/10.1016/0014-5793(85)81063-2
Abstract
Tumour-promoting phorbol esters (phorbol-12-myristate-13-acetate, PMA; phorbol-12,13-dibutyrate, PDBu) but not 4β-phorbol, activate protein kinase C. Using human platelets pre-labelled with quin2 or 32PO4 we examined the effects of these compounds on human platelet cytosolic free Ca2+ ([Ca2+]j) and on [32]phosphatidic acid ([32P]PtdOH). PMA and PDBu, but not 4β-phorbol inhibited thrombin-, PAF- and vasopressin-induced elevation of [Ca2+], and [2+P]PtdOH formation. It is suggested that protein kinase C may act to terminate the transduction processes that link receptor occupancy to cellular activation.Keywords
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