Glucosinolate Biosynthesis
- 1 February 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 86 (2), 319-321
- https://doi.org/10.1104/pp.86.2.319
Abstract
After removal of myrosinase activity by concanavalin A-Sepharose 4B chromatography, cell-free extracts of light-grown cress (Lepidium sativum L.) seedlings, catalyzed the sulfation of desulfobenzylglucosinolate (Km, 0.23 millimolar) to benzylglucosinolate using PAPS (Km, 1 millimolar) as sulfur donor. Sulfotransferase activity, which was optimal at pH 9.0, was stimulated by MgCl2, MnCl2, .beta.-mercaptoethanol, and dithiothreitol and was inhibited by ZnSO4 and SH-reagents. The enzyme also sulfated desulfoallylglucosinolate to allylglucosinolate (sinigrin) but was inactive towards all phenylpropanoids and flavonoids tested.This publication has 11 references indexed in Scilit:
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