Identification of IgE‐binding epitopes on gliadins for patients with food allergy to wheat
- 6 May 2005
- Vol. 60 (6), 815-821
- https://doi.org/10.1111/j.1398-9995.2005.00795.x
Abstract
Background: Food allergy to wheat induces different symptoms as atopic eczema/dermatitis syndrome (AEDS), urticaria and more severe reactions as wheat‐dependent exercise‐induced anaphylaxis (WDEIA). Different gliadin classes are involved in this allergy but IgE‐binding epitopes are known only on ω5‐gliadins and for WDEIA cases. Objectives: The aim of the study was to identify IgE‐binding epitopes on several gliadin classes and for several patients with different symptoms and ages. Methods: Eleven sera were analysed by pepscan with overlapping synthetic peptides. Results: Sera from five patients with anaphylaxis, urticaria or WDEIA, displayed strong IgE‐binding to sequential epitopes of the repetitive domains of αβ, γ, ω2 or ω5‐gliadins with two immunodominant epitopes on ω5‐gliadin and a consensus motif of the type QQX1PX2QQ (X1 being L, F, S or I and X2 Q, E or G). One patient allergic to deamidated wheat proteins also had IgE to a repetitive peptide of γ and ω2‐gliadins of the type QPQQPFP. Sera from four patients with AEDS detected no linear epitopes on gliadins, despite the fact that they contained specific IgE to α, β, γ or ω‐gliadins. One child with AEDS recognized cysteine‐containing sequences in the nonrepetitive domains of αβ and γ‐gliadins. Conclusion: B epitopes in wheat allergy were different from B epitopes of coeliac disease. Differences exist in IgE‐binding epitopes between patients with food allergy to wheat. IgE from those suffering from WDEIA, anaphylaxis and urticaria detected sequential epitopes in the repetitive domain of gliadins whereas IgE from AEDS patients probably recognized conformational epitopes.Keywords
This publication has 36 references indexed in Scilit:
- Concurrent cereal allergy in children with cow's milk allergy manifested with atopic dermatitisClinical and Experimental Allergy, 2003
- Update on wheat hypersensitivityCurrent Opinion in Allergy and Clinical Immunology, 2003
- Structural Basis for Gluten Intolerance in Celiac SprueScience, 2002
- A revised nomenclature for allergy: An EAACI position statement from the EAACI nomenclature task forceAllergy, 2001
- A revised nomenclature for allergy: An EAACI position statement from the EAACI nomenclature task forceAllergy, 2001
- A Major Wheat Allergen Has a Gln-Gln-Gln-Pro-Pro Motif Identified as an IgE-Binding EpitopeBiochemical and Biophysical Research Communications, 1996
- Plant Storage ProteinsBiological Reviews, 1995
- Severity Scoring of Atopic Dermatitis: The SCORAD IndexDermatology, 1993
- Skin Test in Diagnosis of Food AllergyAllergy and Asthma Proceedings, 1991
- N-terminal amino acid sequences of ω-gliadins and ω-secalinsBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983