Action of calcium ions on spinach (Spinacia oleracea) chloroplast fructose bisphosphatase and other enzymes of the Calvin cycle
- 15 June 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 188 (3), 775-779
- https://doi.org/10.1042/bj1880775
Abstract
Thiol-treated spinach (Spinacia oleracea) chloroplast fructose bisphosphatase is powerfully inhibited by Ca2+ non-competitively with respect to its substrate, fructose 1,6-bisphosphate. 500 microM-Ca2+ causes virtually complete inhibition and the Ki is 40 microM. Severe inhibition of sedoheptulose bisphosphatase is also caused by Ca2+. A role for Ca2+ in regulation of the Calvin cycle in spinach chloroplasts is proposed.This publication has 12 references indexed in Scilit:
- Mg2+ Translocation across the Thylakoid Membrane: Studies Using the Ionophore A 23187European Journal of Biochemistry, 1978
- An improved method for isolating chloroplasts retaining their outer membranesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977
- Light-induced movement of magnesium ions in intact chloroplasts. Spectroscopic determination with Eriochrome Blue SEBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977
- The effect of the ionophore A23187 on Mg2+ and Ca2+ movements and internal pH of isolated intact chloroplastsPlant Science Letters, 1977
- Light-dependent changes of the Mg2+ concentration in the stroma in relation to the Mg2+ dependency of CO2 fixation in intact chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- Effect of ionophores A23187 and nigericin on the light-induced redistribution of Mg2+, K+ and H+ across the thylakoid membraneBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- Some effects of inorganic phosphate on O2 evolution by isolated chloroplastsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1967
- The reductive pentose phosphate cycle. II. Specific C-1 phosphatases for fructose 1,6-diphosphate and sedoheptulose 1,7-diphosphateArchives of Biochemistry and Biophysics, 1958
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934