Potent vasoconstriction of the isolated perfused rat kidney by leukotrienes C4 and D4

Abstract

Chemically synthesized leukotriene C₄, D₄, and E₄ have been compared for their effects on the isolated Krebs-perfused rat kidney, rat stomach strip, and guinea pig ileum. C₄ was more potent than D₄ or E₄ at all concentrations tested in contracting the rat stomach strip and in constricting the isolated rat kidney, while D₄ was more potent than C₄ or E₄ in contracting the guinea pig ileum. While the effect of leukotrienes on the isolated kidney was blocked dose dependantly by FPL 55712, a blocker of leukotriene action, it was not blocked by the presence of either indomethacin, a cyclooxygenase blocker, or OKY-1581, a blocker of thromboxane synthesis. These results indicate that leukotriene action in the kidney is of a direct nature and is not mediated via activation of the prostaglandin pathway, especially thromboxane A₂ synthesis.