Production of an Inactive Derivative of Purified Prothrombin by Means of Purified Thrombin.

Abstract

When purified prothrombin was allowed to react with a small amt. of purified thrombin there was loss in prothrombin activity. Comparison of the electrophoretic patterns of the prothrombin before and after alteration with thrombin showed that protein disappeared from the curve representing prothrombin and appeared as a new component with a lower electrophoretic mobility than that of prothrombin.