The poliovirus 2C cis-acting replication element-mediated uridylylation of VPg is not required for synthesis of negative-sense genomes
Open Access
- 1 September 2003
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 84 (9), 2359-2363
- https://doi.org/10.1099/vir.0.19132-0
Abstract
Nucleotides in the terminal loop of the poliovirus 2C cis-acting replication element (2CCRE), a 61 nt structured RNA, function as the template for the addition of two uridylate (U) residues to the viral protein VPg. This uridylylation reaction leads to the formation of VPgpUpU, which is used by the viral RNA polymerase as a nucleotide–peptide primer for genome replication. Although VPg primes both positive- and negative-strand replication, the specific requirement for 2CCRE-mediated uridylylation for one or both events has not been demonstrated. We have used a cell-free in vitro translation and replication reaction to demonstrate that 2CCRE is not required for the initiation of the negative-sense strand, which is synthesized in the absence of 2CCRE-mediated VPgpUpU formation. We propose that the 3′ poly(A) tail could serve as the template for the formation of a VPg–poly(U) primer that functions in the initiation of negative-sense strands.Keywords
This publication has 22 references indexed in Scilit:
- Structure and function analysis of the poliovirus cis-acting replication element (CRE)RNA, 2003
- Biochemical and Genetic Studies of the Initiation of Human Rhinovirus 2 RNA Replication: Identification of a cis -Replicating Element in the Coding Sequence of 2A proJournal of Virology, 2001
- Phenotypic Characterization of Three Phylogenetically Conserved Stem-Loop Motifs in the Mengovirus 3′ Untranslated RegionJournal of Virology, 2001
- 5′ cloverleaf in poliovirus RNA is a cis-acting replication element required for negative-strand synthesisThe EMBO Journal, 2001
- Poliovirus Requires a Precise 5′ End for Efficient Positive-Strand RNA SynthesisJournal of Virology, 2000
- Identification of a cis -Acting Replication Element within the Poliovirus Coding RegionJournal of Virology, 2000
- Poliovirus RNA-dependent RNA Polymerase (3Dpol)Published by Elsevier ,1999
- Intracellular determinants of picornavirus replicationTrends in Microbiology, 1999
- [3] Assays for poliovirus polymerase, 3DPol, and authentic RNA replication in HeLa S10 extractsPublished by Elsevier ,1996
- Localization of binding site for encephalomyocarditis virus RNA polymerase in the 3′-noncoding region of the viral RNANucleic Acids Research, 1995