Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock protein
Open Access
- 16 July 2009
- journal article
- research article
- Published by Springer Nature in Genetic Vaccines and Therapy
- Vol. 7 (1), 11
- https://doi.org/10.1186/1479-0556-7-11
Abstract
Background: Protein-calorie malnutrition (PCM) is the most common type of malnutrition. PCM leads to immunodeficiency and consequent increased susceptibility to infectious agents. In addition, responses to prophylactic vaccines depend on nutritional status. This study aims to evaluate the ability of undernourished mice to mount an immune response to a genetic vaccine (pVAXhsp65) against tuberculosis, containing the gene coding for the heat shock protein 65 from mycobacteria.Methods: Young adult female BALB/c mice were fedad libitumor with 80% of the amount of food consumed by a normal diet group. We initially characterized a mice model of dietary restriction by determining body and spleen weights, hematological parameters and histopathological changes in lymphoid organs. The ability of splenic cells to produce IFN-gamma and IL-4 uponin vitrostimulation with LPS orS. aureusand the serum titer of specific IgG1 and IgG2a anti-hsp65 antibodies after intramuscular immunization with pVAXhsp65 was then tested.Results: Dietary restriction significantly decreased body and spleen weights and also the total lymphocyte count in blood. This restriction also determined a striking atrophy in lymphoid organs as spleen, thymus and lymphoid tissue associated with the small intestine. Specific antibodies were not detected in mice submitted to dietary restriction whereas the well nourished animals produced significant levels of both, IgG1 and IgG2a anti-hsp65.Conclusion: 20% restriction in food intake deeply compromised humoral immunity induced by a genetic vaccine, alerting, therefore, for the relevance of the nutritional condition in vaccination programs based on these kinds of constructs.Keywords
This publication has 36 references indexed in Scilit:
- Heat-shock proteins can promote as well as regulate autoimmunityAutoimmunity Reviews, 2008
- Protection against tuberculosis by a single intranasal administration of DNA-hsp65 vaccine complexed with cationic liposomesBMC Immunology, 2008
- Pentamer is the minimum structure for oligomannosylpeptoids to bind to concanavalin ABioorganic & Medicinal Chemistry Letters, 2007
- Interleukin‐12 regulates natural killer cell‐dependent Propionibacterium acnes‐primed, lipopolysaccharide‐induced liver injuryHepatology Research, 2007
- Blood serum interferon-γ bioactivity is low in weanling mice subjected to acute deficits of energy or both protein and energyBritish Journal of Nutrition, 2007
- Induction of apoptosis in rat lymphocytes by starvationClinical Science, 2006
- Assessment by Flow Cytometry of Cytokine Production in Malnourished ChildrenClinical and Vaccine Immunology, 2005
- The thymus gland is a target in malnutritionEuropean Journal of Clinical Nutrition, 2002
- Undernutrition, infection and immune functionNutrition Research Reviews, 2000
- Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells.The Journal of Experimental Medicine, 1993