Bleeding Syndrome in Thrombin-Injected Rats Deficient in Fibrinogen and Antihemophilic Factor

Abstract

One and 2 hours after intraperitoneal injection of 2500 units of thrombin into rats, bleeding from severed mesenteric blood vessels was not arrested and platelet plugs rarely formed. Other manifestations of hemorrhagic tendency were present. Capillary resistance was not diminished. Fibrinogen concentration was low; whole clot fibrinolysis was occasionally observed; prothrombin, factor V, factor VII and platelets were somewhat reduced; PTC concentration was normal; and AHF concentration approached zero. It is suggested that the defect in hemostasis is predominantly the result of AHF deficiency.