The LIM Domain Protein LPP Is a Coactivator for the ETS Domain Transcription Factor PEA3
Open Access
- 1 June 2006
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 26 (12), 4529-4538
- https://doi.org/10.1128/mcb.01667-05
Abstract
PEA3 is a member of a subfamily of ETS domain transcription factors which is regulated by a number of signaling cascades, including the mitogen-activated protein (MAP) kinase pathways. PEA3 activates gene expression and is thought to play an important role in promoting tumor metastasis and also in neuronal development. Here, we have identified the LIM domain protein LPP as a novel coregulatory binding partner for PEA3. LPP has intrinsic transactivation capacity, forms a complex with PEA3, and is found associated with PEA3-regulated promoters. By manipulating LPP levels, we show that it acts to upregulate the transactivation capacity of PEA3. LPP can also functionally interact in a similar manner with the related family member ER81. Thus, we have uncovered a novel nuclear function for the LIM domain protein LPP as a transcriptional coactivator. As LPP continually shuttles between the cell periphery and the nucleus, it represents a potential novel link between cell surface events and changes in gene expression.Keywords
This publication has 45 references indexed in Scilit:
- Transactivation Functions of the Tumor-Specific HMGA2/LPP Fusion Protein Are Augmented by Wild-Type HMGA2Molecular Cancer Research, 2005
- The ETS Domain Transcription Factor Elk-1 Regulates the Expression of Its Partner Protein, SRFJournal of Biological Chemistry, 2005
- A nuclear isoform of the focal adhesion LIM-domain protein Trip6 integrates activating and repressing signals at AP-1- and NF-κB-regulated promotersGenes & Development, 2004
- Zyxin and paxillin proteins: focal adhesion plaque LIM domain proteins go nuclearBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2003
- The Focal Adhesion and Nuclear Targeting Capacity of the LIM-containing Lipoma-preferred Partner (LPP) ProteinJournal of Biological Chemistry, 2003
- Cyclooxygenase-2 Is Overexpressed in HER-2/neu-positive Breast CancerJournal of Biological Chemistry, 2002
- HER2/Neu-mediated activation of the ETS transcription factor ER81 and its target gene MMP-1Oncogene, 2001
- The PEA3 Ets Transcription Factor Comprises Multiple Domains That Regulate Transactivation and DNA BindingPublished by Elsevier ,2001
- PEA3 Is Up-regulated in Response to Wnt1 and Activates the Expression of Cyclooxygenase-2Published by Elsevier ,2001
- Transcriptional Regulation of Human Prostaglandin-endoperoxide Synthase-2 Gene by Lipopolysaccharide and Phorbol Ester in Vascular Endothelial CellsJournal of Biological Chemistry, 1995