Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase
- 1 February 1977
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 2 (1), 51-57
- https://doi.org/10.1007/bf00966021
Abstract
In the present paper we describe the synthesis of Sepharose-boundN-(5′-phosphopyridoxyl)-amino-oxyacetic acid,N-(5′-phosphopyridoxyl)-canaline, andN-(5′-phosphopyridoxyl)-α,γ-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). The three Sepharose-N-(phosphopyridoxyl)-amino acids were capable of binding GAD from a mouse brain soluble preparation. The enzyme can be purified up to 25-fold in one step by affinity chromatography on Seph-DAB-PLP.Keywords
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