RAPID PRODUCTION OF COMPLEMENT FIXATION ANTIGENS FOR SYSTEMIC MYCOTIC DISEASES I

Abstract

Five different media were tested for their ability to produce complement-fixation antigens from Coccidioides immitis. The cultures were incubated while stationary at 25[degree]C or were continuously agitated at 25[degree]C for 1 to 4 weeks. A neopeptone dialyzate medium with agitation produced the most satisfactory antigen, after 3 weeks of growth. It was as antigenic as the standard coccidioidin, free of anticomplementary activity, and was readily reproducible. This complement-fixation antigen lacked skin test reactivity.