DIFFERENTIAL FLUORESCENCE OF SISTER CHROMATIDS WITH 4′-6-DIAMIDINO-2-PHENYLINDOLE
- 1 September 1976
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Genetics and Cytology
- Vol. 18 (3), 545-547
- https://doi.org/10.1139/g76-068
Abstract
Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4′-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.Keywords
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