Isolierung des kristallinen Hauptanteils aus Seidenfibroin. Vorläufige Mitteilung

Abstract

Silk is treated with papain, then dissolved in 50% lithium rhodanide and dialyzed. The resulting silk fibroin is treated with crystalline chymotrypsin. This leads to the precipitation of a crystalline peptide containing tyrosine, alanine, serine and glycine in the ratio 1:1:2:3. The amino end group belongs to glycine. The substance is hydrolzed by papain if lithium rhodanide is present. Acid hydrolysis leads to the formation of 3 peptides containing glycine and serine, glycine and tyrosine, and glycine, serine and alanine, respectively.