The Intracellular Concentration of Pyrophosphate in the Batch Culture of Escherichia coli

Abstract

We applied our colorimetric PP_i determination method [Heinonen, J., Honkasalo, S. and Kukko, E. (1981) Anal. Biochem. 117, 293–300] to bacterial cultures and measured the intracellular concentration of PP_i in the batch culture of Escherichia coli. The cells growing in minimal medium contained about 2.5 nmol PP_i/mg protein (0.5 mM). There was no extracellular PP_i. A similar concentration of PP_i was found also in cells growing in minimal medium enriched by amino acid mixture.